The effect of chitin on the biological activity of Bacillus subtilis strains
Keywords:biological control, Vitaplan, fungistatic activity, antagonistic effect, chitosan, chitin, induced resistance, Triticum aestivum, Cochliobolus sativus, Puccinia recondita
The aim of the work was to assess the effect of various forms of chitin and chitosan during submerged cultivation of Bacillus subtilis strains, which form the basis of the laboratory sample Vitaplan, CL, on the synthesis of chitinase, as well as on the antagonistic activity and inducing effect of B. subtilis strains in the pathosystems of wheat - Cochliobolus sativus and Puccinia recondita f. sp. tritici. The inclusion of chitin in the form of dry powder or chitin and chitosan in the form of a colloidal suspension into the medium for deep cultivation of bacteria showed that only colloidal chitin increased the antagonistic activity of B. subtilis strains against test cultures of Alternaria solani and Clavibacter michiganensis. The ability of B. subtilis strains to synthesize extracellular chitinase when cultivated in a medium containing colloidal chitin was established. A higher fungistatic effect of the laboratory sample Vitaplan CL + colloidal chitin against Cohliobolus sativus was revealed as compared to the original sample. It was shown that the laboratory sample Vitaplan, CL + colloidal chitin increases the resistance of wheat to dark brown spot and brown rust 1.5-2.0 times more effectively as compared to Vitaplan, CL. As a result of the research, a laboratory sample of Vitaplan, CL + colloidal chitin was obtained with increased antagonistic and inducing activity as compared to Vitaplan, CL.
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