PYRAMIDING OF PATHOGEN RESISTANCE GENES VIA CROSSING OF MEXICAN POTATO SPECIES SOLANUM NEOANTIPOVICZII WITH SELECTION FROM ‘AURORA’ VARIETY
AbstractIn this paper we have studied the potato plants from a hybrid population obtained by crossing of the Mexican species Solanum neoantipoviczii sample, combining high resistance to late blight (gene R2-like) and Potato Virus Y (PVY) (gene Rysto), with the variety Aurora selection. The Aurora selection possesses the identified markers of genes responsible for resistance to nematode (Globodera rostochiensis) of Ro1 pathotype (gene H1) and to late blight (gene R1). Genotyping data of this crossing combination demonstrates the availability of the pathogen resistance gene pyramiding when crossing components complementing each other according to the detected markers. In the obtained hybrid population, 4 gene markers conferring resistance to Phytophthora infestans, PVY and nematode were detected in a half of the plants; two of them diagnose late blight resistance. Plant population has been artificially infected by treating them with two isolates of P. infestans and different inoculum concentrations. When applying extremely high inoculum concentration, the plants with detected markers of two genes with resistance to late blight have showed high resistance, accompanied by a hypersensitivity reaction.In this research, on original plant material, we have demonstrated the ability to develop the potato plant genotypes with gene cassettes via an introgressive hybridization using DNA-markers.
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