Molecular-genetic approaches to studying wheat leaf rust populations
Keywords:Puccinia triticina, RAPD, SNP, SSR marker, virulence, Lr-gene
Population studies of the leaf rust agent Puccinia triticina Erikss. have a long-time history. This review describes the traditional phytopathological and modern molecular methodological approaches used in P. triticina studies. The results of the analysis of P. triticina Russian populations according with virulence and intrapopulation and interpopulation diversity studies of the pathogen by DNA polymorphism are summarized. The advantages and disadvantages of RAPD, AFLP and SSR analyses for studies of P. triticina populations are discussed. The original results of analysis of P. triticina populations using RAPD and SSR markers are presented. Since the middle of 2010, new SNP markers have been developed for coevolution studies in P. triticina populations existed on the common and durum wheats. The possibility and perspective of using a new methodological approach for assessing the degree of phylogenetic relationship between Derbent isolates of P. triticina obtained from Triticum sp. and Aegilops sp. different ploidy are discussed. Prospects for the creation and practical use of new molecular genetic diagnostic systems for monitoring populations, tracking the emergence of new races, their distribution and migration are presented.